《植物生理学报》 2014, 50(4): 519-526

同源四倍体铁皮石斛的生长及多糖积累江金兰

江金兰^1,*, 叶炜^1,*, 李永清¹, 周建金¹, 雷伏贵¹, 魏道智^2,**

¹三明市农业科学研究院药用植物研究所, 福建沙县365509; ²福建农林大学生命科学学院, 福州350002

通信作者：江金兰；E-mail: weidz888@sohu.com；Tel: 0598-5862594

摘　要：

通过秋水仙素诱导铁皮石斛多倍体, 以外观形态、气孔、染色体数目筛选多倍体植株, 分析多倍化对铁皮石斛多糖累积及生长的影响。结果表明, 同源四倍体铁皮石斛茎、叶及原球茎的多糖含量分别是二倍体的1.31、1.83及1.95倍, 但生长速度显著或极显著低于对照。ISSR分析显示, 同源四倍体植株产生较明显的基因变异。

关键词：铁皮石斛; 多倍体; ISSR; 多糖

收稿：2013-12-17   修定：2014-02-26

资助：三明市科技计划项目(2011-K-1)和三明市农业科学研究院院立项目(2013-Y-01)。

Growth and Polysaccharides Accumulation in Autotetraploid Dendrobium officinale

JIANG Jin-Lan^1,*, YE Wei^1,*, LI Yong-Qing¹, ZHOU Jian-Jin¹, LEI Fu-Gui¹, WEI Dao-Zhi^2,**

¹The Institute of Medicinal Plant, Sanming Academy of Agricultural Science, Shaxian, Fujian, 365509, China; ²College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Corresponding author: JIANG Jin-Lan; E-mail: weidz888@sohu.com; Tel: 0598-5862594

Abstract:

Polyploidy Dendrobium officinale was induced by colchicine. Autotetraploid plantlets were screened by appearance, stoma and chromosome number. The accumulation of polysaccharides and growth rate in autotetraploid were also analyzed. Results indicated that, the contents of polysaccharides in stem, leaf, and protocorm- like body of autotetraploid were 1.31, 1.83 and 1.95 fold to diploid, but the growth rate were significantly or very significantly lower than the control. Obviously genomic mutations were observed by ISSR molecular marker analysis in autotetraploid plants.

Key words: Dendrobium officinale; polyploid; ISSR; polysaccharide